- #How to reference imagej software in a paper manual
- #How to reference imagej software in a paper Patch
#How to reference imagej software in a paper manual
Outcomes were virtually identical to the current gold standards for contraction measurement such as optical flow, pole deflection, edge-detection systems or manual analyses.
#How to reference imagej software in a paper Patch
MUSCLEMOTION was effective under different recording conditions (bright field microscopy with simultaneous patch clamp recording, phase contrast microscopy and traction force microscopy). Methods and Results: MUSCLEMOTION allowed rapid and easy measurement of movement from high-speed movies in: (i) 1-dimensional in vitro models such as isolated adult and human pluripotent stem cell-derived CMs (hPSC-CMs) (ii) 2-dimensional in vitro models, such as beating CM monolayers or small clusters of hPSC-CMs (iii) 3-dimensional multicellular in vitro or in vivo contractile tissues such as cardiac "organoids", engineered heart tissues (EHT), zebrafish- and human hearts. Objective: Here we describe and validate an automated, open source software tool (MUSCLEMOTION) adaptable for use with standard laboratory- and clinical imaging equipment that enables quantitative analysis of normal cardiac contraction, disease phenotypes and pharmacological responses. Consequently, data and techniques have been difficult to reproduce across models and laboratories, analysis is time consuming and only specialist researchers can quantify data. Rationale: There are several methods to measure cardiomyocyte (CM) and muscle contraction but these require customized hardware, expensive apparatus and advanced informatics or can only be used in single experimental models. Easy to use for anybody with a microscope and camera. The Leiden University Medical Center and Glasgow University by a combination ofīasic scientists, engineers and medical doctors, we aim to provide a simple software tool to quantify contraction without the need for detailed knowledge of software or expensiveĮquipment. Originally designed for quantifying 2D stemĬell derived cardiomyocyte contraction, it proved applicable to many differentĬell types, systems and experimental setups. MUSCLEMOTION is a powerful and versatile ImageJ macro to measure in vitro or in Further details can be found on the original publication on Circulation Research.Ī detailed step-by-step protocol description can be found on the protocol paper on Current Protocols in Human Genetics. Here below you will find a brief description of MUSCLEMOTION. MUSCLEMOTION Open-source ImageJ plugin to quantify cardiomyocyte and cardiac muscle contraction in vitro and in vivo
0 kommentar(er)
